Green Tea and Red Tea from Camellia sinensis Partially Prevented the Motor Deficits and Striatal Oxidative Damage Induced by Hemorrhagic Stroke in Rats.

Priscila Marques Sosa,Mauren Assis De Souza,Pâmela B Mello-Carpes

doi:10.1155/2018/5158724

Abstract

Green tea from Camellia sinensis plays a well-established neuroprotective role in several neurodegenerative diseases, including intracerebral hemorrhage (ICH). However, the other teas of the same plant do not have their properties well understood; but they can be as effective as green tea as an alternative therapy. In this study, we investigated the effects of supplementation with green tea and red tea from Camellia sinensis on motor deficits and striatum oxidative damage in rats submitted to hemorrhagic stroke (ICH). Male Wistar rats were supplemented with green tea, red tea, or vehicle for 10 days prior to ICH induction. After injury, the rats were submitted to motor tests (open field for locomotion, rotarod for balance, and neurological deficit scale (NDS)) 1, 3, and 7 days after ICH induction, while the tea supplementation was maintained. Subsequently, the rats were euthanized to striatal tissue dissection for biochemical analyzes (lipid peroxidation, reactive oxygen species, glutathione levels, and total antioxidant capacity). ICH caused locomotor and balance deficits, as well as increased the neurological deficit (NDS). Only red tea prevented locomotor deficits after injury. Green tea and red tea prevented balance deficits on the seventh day after ICH. On NDS evaluation, green tea presented a better neuroprotection than red tea (until day 3 after ICH injury). In addition, ICH increased reactive oxygen species and lipid peroxidation levels, without altering antioxidant markers. Green and red teas were effective in decreasing the lipid peroxidation levels. Therefore, green and red teas partially prevented the motor deficits and striatal oxidative damage induced by ICH. Based on our results, we can consider that the two teas seem to be equally effective to prevent motor deficits and striatal oxidative damage induced by hemorrhagic stroke in rats.

Highlights

Intracerebral hemorrhage (ICH) is a common type of stroke associated with a considerable socioeconomic impact, disability, and mortality [1] and represents 15 to 20% of all stroke cases [2].Some regions of the brain are more susceptible to stroke damage, including striatum, which is one of the most important regions for voluntary motor control [3]
We determined the neuroprotective potential of green tea and red tea on the motor deficits and striatal damage caused by hemorrhagic stroke in rats
A significant finding is that on the seventh day after the injury, a spontaneous recovery was observed in relation to the locomotor and exploratory activity, which is in agreement with Lu et al [29], but prevents us from evaluating whether the teas have a protective effect in this case

Summary

Introduction

Intracerebral hemorrhage (ICH) is a common type of stroke associated with a considerable socioeconomic impact, disability, and mortality [1] and represents 15 to 20% of all stroke cases [2].Some regions of the brain are more susceptible to stroke damage, including striatum, which is one of the most important regions for voluntary motor control [3]. Progressive deterioration of brain tissues is classified as ICH secondary damage and plays an important role in neurological impairment [4]. These molecular events that occur during ICH increase the production of hydroxyl radicals and oxidation of lipids [6], which expose the brain to higher levels of reactive oxygen species (ROS). There is increasing evidence that oxidative stress contributes to ICH-induced secondary brain injury through the generation of ROS [7]. The biochemical events involved in secondary damage are not well described but represent an important therapeutic target after ICH [7]

Methods

Results

Conclusion