Green synthesized silver nanoparticles using Andrographis macrobotrys Nees leaf extract and its potential to antibacterial, antioxidant, anti-inflammatory and lung cancer cells cytotoxicity effects

Abstract

Green silver nanoparticles have received much interest over the years because they are cheap, good for the environment, and easy to use. Present study, first report to synthesized silver nanoparticles from the leaf extract of Andrographis macrobotrys, which reduces AgNO3 into Ag through the presence of phytochemicals. The nanoparticles were examined using (UV, spec, FTIR, XRD, TEM and EDAX. The dark brown colour of the A. macrobotrys colloidal showed maximum absorbance at 450 nm. The TEM images displayed synthesised nanoparticles size were revealed between 20 and 50 nm. The antibacterial activity of Ag-NPs tested show a maximum zone of inhibition of 19 mm for Escherichia coli and Staphylococcus aureus 17 mm for at 125 µg/mL. Green synthesized AgNPs were assessed for antioxidant activity inhibition rate (DPPH 58.23 % and ABTS 68.87 %). Further, the anticancer activity of AgNPs exhibited 68.15 % at 100 µg/mL concentration against A549 lung cancer cells. Additionally, in vitro models using the human red blood cells (HRBC) membrane stabilisation method (MSM) were used to assess the anti-inflammatory effects of AgNPs of A. macrobotrys and its shown to have a MSM of 76.6 % at a dosage of 250 µg/mL. A. macrobotrys derived AgNPs possess multi potential activity was used in future pharmaceutical applications.