Green Synthesis of Water-Compatible Fluorescent Molecularly Imprinted Polymeric Nanoparticles for Efficient Detection of Paracetamol

Abstract

In this work, one-pot green synthesis of a novel kind of water-compatible fluorescent molecularly imprinted polymer nanoparticle for selective optosensing of paracetamol was developed via macromolecular assembly of an amphiphilic fluorescent copolymer and in situ photo-cross-linking. Amphiphilic photo-cross-linkable and fluorescent copolymers containing carbazole groups were synthesized and could coassemble with paracetamol (PCM, template molecule) and photoinitiator in aqueous solution. The obtained photo-cross-linkable fluorescent nanoparticles were then cross-linked triggered by UV-irradiation, generating the hydrophilic and fluorescent molecularly imprinted polymer nanoparticles (FMIP nanoparticles) in aqueous media. The whole procedure was carried out at mild working condition, which is facile, green, and energy-saving. The resulting FMIP nanoparticles showed high selectivity toward PCM and obvious fluorescence quenching, induced by template-binding, in water and were a kind of efficient fluorescent chemosensor for the sensing of PCM. A wide linear range over PCM concentration from 4 to 1000 μM with a detection limit of 1.0 μM has been demonstrated using FMIP nanoparticles as chemosensor. Moreover, a rapid response of less than 2 min has been demonstrated. Finally, such a chemosensor based on FMIP nanoparticles was also successfully employed for the detection of PCM in commercial PCM tablets as well as urine samples.