Green synthesis of Silver Nanoparticles using Premna tomentosa leaf extract and its effective antibacterial activity

Abstract

Green synthesis of silver nanoparticles was done at room temperature using Premna tomentosa leaf extract. Silver nanoparticle formation was preliminarily confirmed through UV Spectroscopy and characterized by FTIR, XRD, TEM and SEM. The λmax was observed at 424nm in UV spectroscopy results. The XRD and EDAX spectrum confirmed the presence of silver ions and the crystalline nature of synthesized silver nanoparticles. The synthesized silver nanoparticles were spherical, hexagonal and irregular in shape. FTIR results have revealed the functional groups present in the synthesized nanoparticles such as C-N stretch, N-O symmetric stretch, C-H stretch, N-H bend, C-H group, O-H stretch and H bond. TEM and SEM results showed a clear image of nanoparticles in the size range 100nm and 200nm. In the application part, we focused on the antibacterial activity of synthesized nanoparticles on six pathogens namely Escherichia coli, Bacillus subtilis, Staphylococcus aureus, Pseudomonas aeruginosa, Vibrio parahaemolyticus and Proteus mirabilis. The maximum zone of inhibition was found in Vibrio parahaemolyticus followed by Staphylococcus aureus and Pseudomonas aeruginosa.