Abstract
Purpose: To synthesize silver nanoparticles (AgNPs) of Arbutus andrachne leaf water extract (LE) and to evaluate the antimicrobial activity of both LE and AgNPs.Methods: The synthesized AgNPs were characterized using the following techniques: ultraviolet-visible spectroscopy (UV-vis), Fourier transform infrared spectroscopy (FT-IR), transmission electron microscopy (TEM), thermal gravimetric analysis (TGA), X-ray diffraction (XRD) analysis, and analysis of particle size (PS) and zeta potential (ZP). The antimicrobial activities of LE and NPs were assessed by Kirby-Bauer disc diffusion (DD) and broth microdilution (MD) methods according to the recommendations of the Clinical and Laboratory Standards Institute (CLSI). LE and AgNPs were examined against fresh cultures of four Gram-positive and five Gram-negative bacteria, and three yeast strains.Results: AgNPs were successfully synthesized and characterized using Arbutus andrachne LE. The AgNPs showed moderate antibacterial activity against Staphylococcus aureus ATCC 6538p, S.epidermidis ATCC 12228, Escherichia coli ATCC 29998, Klebsiella pnemoniae ATCC 13883 and Pseudomonas aeruginosa ATCC 27853, and also antifungal activity against Candida albicans ATCC 10239 and C. krusei ATCC 6258.Conclusions: Due to the potent activity of AgNPs against Gram-positive and Gram-negative bacteria, and yeast strains, it is suggested that AgNPs are potential broad spectrum antimicrobial agents.Keywords: Arbutus andrachne, Silver nanoparticles, Antimicrobial activity
Highlights
Nanotechnology is attracting growing interest due to its application in various fields such as medicine, biotechnology and energy consumption [1]
The color of the bionanoparticles prepared by silver nitrate and leaf water extract (LE) (AgNO3/A. andrachne extract) changed from yellow to gray-brown after incubation for 30 min at 50 oC
A reduction of the aqueous Ag+ ions during exposure to the broth of boiled A. andrachne leaves may be followed by ultraviolet-visible spectroscopy (UV-vis) spectroscopy (Figure 1)
Summary
Nanotechnology is attracting growing interest due to its application in various fields such as medicine, biotechnology and energy consumption [1]. Nanoparticle synthesis is often used in applications such as pyrolysis, laser ablation, vapor deposition, sol gel and lithography electro-deposition [2]. Green nanoparticle synthesis from plant extracts is easy, relevant, efficient and fast compared to chemical and physical methods [3]. The synthesis of nanoparticles by biological methods using microorganisms or plant extracts has been suggested as an eco-friendly alternative [4]. Gold nanoparticles – and silver nanoparticles (AgNPs) – are of particular interest in biology, chemistry and physics due to their optical, mechanical and electronic properties [5,6]. Silver is the most important metal in the synthesis of bionanoparticles because it is an antimicrobial agent that protects against the increasing threat posed by antibiotic resistant microbes [7]
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