Abstract

Biologically the silver nanoparticles were synthesized from Indigofera cordifolia leaves extract. The absorbance of the silver nanoparticles centered at four hundred and twenty nm, with respect to the surface plasmon resonance of silver nanoparticles wavelength. XRD method proves, biosynthesized NPs would retain the face centered cubic (fcc) structure. In TEM image analysis, silver NPs morphology was spherical in shape. The composition of the silver nanoparticles was obtained by EDAX analysis method. FTIR analysis concluded that biosynthesis Ag NPs was observed at 1384 cm-1, with respect to –NO3 stretching arises from AgNO3. Ten types of bands are present in the broad emission because of organic matrix bound to silver nanoparticles, which reveals as the result of photoluminescence measurements. The silver NPs possess more antibacterial activity as compared to the standard drug, Amoxicillin.

Highlights

  • The green synthesis methods which are eco friendly methods in the field of science and technologies are very much popular in the future and these methods are essential to minimize the issues in agriculture (Thuesombat et al, 2014)

  • The antimicrobial activity of the AgNPs were determined by the well diffusion assay on MHA towards Klebsiella pneumonia, Streptococcus aureus, Shigella dysenteriae, Escherichia coli, Pseudomonas aeruginosa, Staphylococcus pneumonia and Pseudomonas vulgaris

  • Ag NPs were produced from Indigofera cordifolia leaf extract using the Green method

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Summary

Introduction

The green synthesis methods which are eco friendly methods in the field of science and technologies are very much popular in the future and these methods are essential to minimize the issues in agriculture (Thuesombat et al, 2014). It is a subshrub, originate in moist deciduous forests and in plains, the plant is widely cultivated (Sasidharan, 2004). The flavonoid fraction of Indigofera tinctoria had chemopreventive effect against benzeo pyrene induced lung cancer (Ravichandran & Ravichandran, 2008). The tangential analgesic possessions of Indigofera tinctoria was reported Saravanakumar, 2009. The methanolic extract of the entire plant possessed antihelmintic activity against Pheretima posthuma (Gunasekaran, et al, 2009). The ethanolic leaf extract Indigofera tinctoria have the ability to inhibit the growth of gram positive bacteria namely Bacillus pumilus, Staphylococcus aureus and Streptococcus pyrogens. The cytotoxic effect of Indigofera tinctoria leaf extract on lung cancer cell line NCIH69 was studied. Present work, AgNPs are synthesized Indigofera cordifolia leaf extract using a green approach.

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