Abstract
The paper reports a green chemistry approach for the synthesis of silver nanoparticles (AgNPs) using hypericin-rich shoot cultures of Hypericum hookerianum as reducing agent. Normal green shoot cultures deficient in hypericin and red-pigmented shoot cultures rich in hypericin (3.01% DW) were raised in Murashige and Skoog nutrient medium containing 1.0 mg/L kinetin (KIN) and 0.2 mg/L naphthaleneacetic acid (NAA), respectively. Dried powder extracts of whole shoots were used for AgNPs formation. The effect of temperature on the formation of AgNPs is investigated. The nanoparticles obtained were characterised using UV–Vis spectroscopy, field emission scanning electron microscopy (FESEM), energy-dispersive X-ray (EDX) and X-ray diffraction (XRD) analyses. The UV–Vis spectra of AgNPs gave surface plasmon resonance (SPR) at 440 nm. The synthesised AgNPs were effective against different multidrug-resistant human pathogens such as Bacillus subtillis (Gram positive) and Pseudomonas aeruginosa (Gram negative) species. Further, the effect of hypericin concentration on anti-bacterial activity was investigated and was found to increase with increase in concentration.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
