Green synthesis of silver nanoparticles using green tea leaf extract, characterization and evaluation of antimicrobial activity.

Abstract

The use of nanoparticles in medicine, nanomedicine, is very important to diagnose and treat diseases; among the various metallic nanoparticles, silver nanoparticles (AgNPs) are very popular due to their physical, chemical, and biological properties, encompassing a range of activities such as antiviral, antifungal, anti-inflammatory, and anticancer activities. In this study, the synthesis of AgNPs was conducted by the use of a nontoxic, ecofriendly method. Green tea (GT) leaf extract was used as a reducing agent to convert silver ions into free AgNPs. The UV-vis spectrum showed a peak at 410 nm, confirming the presence of AgNPs. A Fourier-transform infrared (FTIR) analysis of the GT extract and GT AgNPs display spectra that is identical to those of polyphenols, polysaccharides, and proteins. All the vibrational peaks in the GT extract spectrum were shifted in the AgNP spectrum, becoming narrower after the encapsulation of nanoparticles. The scanning electron microscopy (SEM) images confirm the presence of AgNPs with different sizes, ranging from 15 to 33 nm. Furthermore, the antibacterial activity of the synthesized AgNPs in three different concentrations (10, 20, and 50 mg ml−1) showed appreciable inhibition of bacterial growth against Staphylococcus aureus and Klebsiella sp. From the above findings, we can recommend the use of AgNPs from GT leaf extracts as an antimicrobial agent to treat chronic infections.