Abstract

Synthesis of silver nanoparticles using seeds of Nigella sativa as a capping agent was evaluated in this study. Different concentrations of the aqueous extract of N. sativa with silver nitrate solution were exposed to sunlight; as a force for acceleration of the formulation. Then the silver nanoparticles were characterized by UV-Vis, scanning electron microscope (SEM) and X-ray diffraction (XRD) techniques. Antibacterial activity of the nanoparticles was investigated against Staphylococcus aureus and Escherichia coli by the disc diffusion method. The characterization of nanoparticles was detected by the change in color to yellow-brown which indicated the formulation of silver nanoparticles. Irregular shapes within range of nanoscale were detected using SEM and XRD techniques. The finding suggests that silver nanoparticles may be effectively used as antibacterial agent.

Highlights

  • In today’s world, nanotechnology is a relatively new field, but its structural nanometer dimensions are not new, and these materials have much significance

  • The silver nanoparticles were characterized by UV-Vis, scanning electron microscope (SEM) and X-ray diffraction (XRD) techniques

  • Antibacterial activity of the nanoparticles was investigated against Staphylococcus aureus and Escherichia coli by the disc diffusion method

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Summary

Introduction

In today’s world, nanotechnology is a relatively new field, but its structural nanometer dimensions are not new, and these materials have much significance. There is a continuous increase in the number of multidrug resistant bacterial and viral strains due to mutation, pollution and changing environmental conditions [10] To circumvent this predicament, scientists are trying to develop drugs for the treatment of such microbial infections. The present study was aimed to make an effective approach for synthesizing silver nanoparticles using seeds of Nigella sativa as a reducing agent and to assessing the potential for their use in the treatment of urinary tract infection

Preparation of Extract from Seeds of Nigella sativa
Synthesis of Silver Nanoparticles
Characterization of Silver Nanoparticles
Antimicrobial Activity by Disc Diffusion Method
Antimicrobial Activity
Conclusion

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