Abstract
AbstractThe present study explores the potential of Valeriana jatamansi shoot extract for Ag-metal bio-reduction and its antimicrobial activity. Among the different ratios of AgNO3 and extract tested, 1:5 (1 mL AgNO3 and 5 mL extract) gave maximum SPR peak at 411.0 nm during UV-Vis spectrophotometric analysis, indicating the synthesis of maximum amount of AgNPs in solution. XRD analysis reported the crystalline nature of AgNPs with 13.32 nm nanocrystallite size. FTIR studies suggested the involvement of carboxylic acid (–[C–O–O–H]) and methane (–CH–) functional groups of different compounds in AgNPs reduction and fabrication. Average size of synthesized uniform shaped nanospheres was 32 nm by SEM image analysis. The produced AgNPs (1.5 mg/disc) showed growth inhibition of 71.46, 65.97, 61.5, 55.32, and 54.83% against Pseudomonas aeruginosa, Escherichia coli, Candida albicans, Xanthomonas campestris, and Staphylococcus aureus. While the least growth inhibition of 48.55% was recorded for Klebsiella pneumonia, suggesting it as the least-susceptible microbe among all the tested microbial species. P. aeruginosa was found to be most sensitive of all tested microbes, while E. coli, C. albicans, and X. campestris reported moderate susceptibility to AgNPs.
Highlights
Nanobiotechnology is the intersection of biology and nanotechnology
All the solutions were analyzed by UV-visible (UV-Vis) spectrophotometer, and the solution containing maximum amount of AgNPs was further processed for AgNPs characterization
Antimicrobial potential of AgNPs was assessed against different clinical isolates of Klebsiella pneumonia, Bacillus subtilis, and ATCC strains of Pseudomonas aeruginosa, Staphylococcus aureus, Escherichia coli, Xanthomonas campestris, and Candida albicans by following the protocol described by Bakht et al [16]
Summary
Nanobiotechnology is the intersection of biology and nanotechnology. The development and the use of nanotools like nanoparticles in a vast field to achieve numerous goals have attracted scientists and researchers to this field. Valeriana jatamansi (family Valerianaceae), an indigenous medicinal plant of Himalaya region, is a hairy, perennial dwarf and rhizomatous wild herb. It can be found growing at an altitude of 1,200–3,000 m. Ester iridoids isolated from family Valerianaceae are documented for cytotoxic, sedative, antifungal, and antitumor properties [15]. This present study was designed, owing to medicinal potential of V. jatamansi, to investigate the green synthesis of silver nanoparticles from V. jatamansi shoot extract and its antimicrobial activity. All the solutions were analyzed by UV-visible (UV-Vis) spectrophotometer, and the solution containing maximum amount of AgNPs was further processed for AgNPs characterization
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