Green Synthesis of Silver Nanoparticles by Fusarium oxysporum and its Function Against Aspergillus and Fusarium Fungi.

Abstract

(NPs) can be produced by various methods such as physical and chemical processes. However, environmentally friendly ways are increasingly requested. In this research, (Ag-NPs) were produced by Fusarium oxysporum, and its antifungal effect on Aspergillus and Fusarium was investigated. Nanoparticles were produced by silver nitrate salt and Fusarium oxysporum native to Isfahan city. In order to optimize the synthesis conditions, optimization of some factors such as volume, concentration, time, temperature, and pH of the extract was performed. The structural and physical properties of NPs were determined by spectrophotometer, XRD, FTIR FESEM, SEM, and TEM microscopy. For the study of the inhibitory effect of NPs on Fusarium and Aspergillus growth, the fungi were cultured in media containing various concentrations of NPs from 50 to 1500ppm. Then, the colony diameter was measured for over 10days and the growth inhibition percentage was estimated. For statistical analysis, the 600 Mann-Whitney tests have been applied.The NPs were produced after mixing the powdered fungal mass and silver nitrate salt in optimum conditions which were 2mM of salt, triple fungal mass volume proportion relative to the salt, pH of 9, and temperature of 28°C. The existence of a peak at 420nm in FTIR was due to nanoparticle production. Based on the XRD, the synthesized NPs had suitable properties similar to the standard NPs reported in the studies. Images from TEM, SEM, and FESEM microscopes displayed uniform NPs in variable sizes between 25 and 100nm. According to the results, the maximum growth inhibition percentage of Ag-NPs on Fusarium was approximately 60% at 1500ppm, and 88% on Aspergillus at 800ppm. Biosynthesized Ag-NPs with Fusarium oxysporum have desirable structural traits and can inhibit the growth of Fusarium and Aspergillus at significant levels.