Abstract
The present study focuses on the biological synthesis, characterization, and antibacterial activities of silver nanoparticles (AgNPs) using extracellular extracts of Aspergillus japonicus PJ01.The optimal conditions of the synthesis process were: 10 mL of extracellular extracts, 1 mL of AgNO3 (0.8 mol/L), 4 mL of NaOH solution (1.5 mol/L), 30 °C, and a reaction time of 1 min. The characterizations of AgNPs were tested by UV-visible spectrophotometry, zeta potential, scanning electron microscope (SEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), and thermogravimetric (TG) analyses. Fourier transform infrared spectroscopy (FTIR) analysis showed that Ag+ was reduced by the extracellular extracts, which consisted chiefly of soluble proteins and reducing sugars. In this work, AgNO3 concentration played an important role in the physicochemical properties and antibacterial properties of AgNPs. Under the AgNO3 concentration of 0.2 and 0.8 mol/L, the diameters of AgNPs were 3.8 ± 1.1 and 9.1 ± 2.9 nm, respectively. In addition, smaller-sized AgNPs showed higher antimicrobial properties, and the minimum inhibitory concentration (MIC) values against both E. coli and S. aureus were 0.32 mg/mL.
Highlights
In recent years, mental nanoparticles (MNPs) have been applied widely in many fields, such as catalysis, optics, antimicrobials, etc
In our previous report [7], A. japonicus PJ01 was used for multi-enzyme complexes (MEC) production under solidstate fermentation (SSF), and the MEC contained pectinase, CMCase, and xylanase
In a previous report [7], A. japonicus PJ01 was used for multi-enzyme complexes (MEC) production under solid-state fermentation (SSF)
Summary
Mental nanoparticles (MNPs) have been applied widely in many fields, such as catalysis, optics, antimicrobials, etc. Biogenic synthesis of AgNPs can be performed by using organisms such as bacteria, fungi, plants, or the by-products of their metabolism, which act as reducing and stabilizing agents. Among these organisms, fungi are attractive because they offer high tolerance to metals and are easy to handle. Fungi are attractive because they offer high tolerance to metals and are easy to handle They secrete large quantities of extracellular proteins that contribute to the stability of the nanoparticles [4]. Most fungal extracellular extracts used for biogenic synthesis of AgNPs were derived from SmF; few reports focused on SSF
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