Abstract
Introduction and Aim: The silver nanoparticles have attained a special place in the area of nanotechnology because of their different biological applications. Fabrication of nanoparticles using green synthesis is done because of its wide applications in different fields such as biomedical, medicine, agriculture and food engineering. This study is to develop an easy and eco-friendly method for the synthesis of Ag-NPs using leaf extracts of the medicinal plant.
 Materials and Methods: The medicinal plants are rich sources of various medicinal properties. Olea dioica Roxb., leaf extract was used to investigate the effects of Ag-NPs having antibacterial activity and antioxidant capacity. The plant leaf extract contains flavonoids, alkaloids, saponins, and phenolic compounds which acts as reducing and stabilizing agents. The green synthesized silver nanoparticles were characterized by various techniques like UV- visible spectrophotometer, FTIR spectroscopy, and SEM analysis.
 Results: The synthesis of sliver nanoparticles from plant source, and analysis of nano particles by UV-Vis spectra, SEM and FTIR. The biological evaluations of Ag-NPs indicated an excellent inhibitory efficacy, antioxidant and antimicrobial activity for their future applications in medicine.
 Conclusion: The synthesized silver nanoparticles exhibited potent antioxidant and antimicrobial activities against Gram-positive and Gram-negative bacteria. The silver (Ag-NPs) nanoparticles synthesized by the pot green synthesis method proves its potential use in various medical applications.
 Keywords: Silver nanoparticles; Medicinal plants; Ag-NPs; Olea dioica Roxb.,
Highlights
MethodsOlea dioica Roxb., leaf extract was used to investigate the effects of Ag-NPs having antibacterial activity and antioxidant capacity
Introduction and AimThe silver nanoparticles have attained a special place in the area of nanotechnology because of their different biological applications
The silver (Ag-NPs) nanoparticles synthesized by the pot green synthesis method proves its potential use in various medical applications
Summary
Olea dioica Roxb., leaf extract was used to investigate the effects of Ag-NPs having antibacterial activity and antioxidant capacity. The plant leaf extract contains flavonoids, alkaloids, saponins, and phenolic compounds which acts as reducing and stabilizing agents. Olea dioica Roxb., leaves were collected from Pilikula Mangalore, Dakshina Kannada, Karnataka, India. Collected leaves were washed 3-4 times with tap water and washed with distilled water, and shade dried at room temperature for 23 days and mechanically made a fine powder. Fifty grams of powder was extracted with methanol (12 hours) using Soxhlet extractor. The extracts were evaporated at room temperature and stored in brown bottles at room temperature until screened. This methanolic extract was used for the synthesis of silver nanoparticles
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