Green synthesis of silver-chitosan nanocomposite and their application as antibacterial material

Abstract

It has been studied green synthesis of silver-chitosan nanocomposite using chitosan as a reducing agent as well as a stabilizing agent and NaOH accelerator. This research develops a new strategy for preparation silver-chitosan nanocomposite through gel phase. The silver-chitosan nanocomposite gel is transformed into colloidal by dissolving into chitosan solution. Localized surface plasmon resonance (LSPR) phenomenon of the colloidal silver nanoparticles were investigated using a UV-Vis spectrophotometer. The shape and size of the nanoparticle silver were analyzed using Transmission Electron Microscopy (TEM). Antibacterial activity test of silver-chitosan nanocomposite against E. coli and S. aureus bacteria by disc diffusion method. The formation of silver nanoparticles in the synthesis of silver nanocomposite-chitosan chitosan through the gel phase is characterized by the appearance of LSPRabsorption peak at 402.4 - 414.5 nm. The optimum conditions of silver-chitosan nanocomposite formation were the use of chitosan (1%) of 60 mL, AgNO3 0.07 mmol, NaOH 7 mmol within 18 hours. Based on TEM image of spherical particles with dominated at particle size between 5 - 11 nm. The antibacterial activity of silver-chitosan nanocomposite is high enough that it is potentially used as an antibacterial material for medical applications.