Green Synthesis of Silver and Gold Nanoparticles using Aloe Vera Gel and Determining its Antimicrobial Properties on Nanoparticle Impregnated Cotton Fabric

Abstract

The present work explores the novel approach towards bio-reduction of metals using Aloe vera extract as a reducing and stabilizing agent. The reactions were conducted at a constant temperature of 32±2°C. The formed silver and gold nanoparticles exhibited maximum absorbance at 420 and 530 nm. A prominent rise in absorbance value was noticed within 15 min during the synthesis of silver and gold nanoparticles. At the end 10 and 16 h a stable absorbance value confirmed the completion of silver and gold nanoaprticles synthesis. The formation of nanoparticles was confirmed based on colour change of solution and its surface plasmon resonance peak measured using UV-Vis Spectrophotometer. The shape and size of nanoparticles were determined using transmission electron microscope, and its crystalline structure by x-ray diffraction studies. The results showed that size of silver and gold nanoparticles were 12 to 40 nm and less than 15 nm, with fcc structure. The formed silver and gold nanoparticles were coated on cotton fabric and tested against Escherichia coli and Staphylococcus aureus.