GREEN SYNTHESIS OF REDUCED GRAPHENE OXIDE SILVER NANOCOMPOSITE USING Anisomeles malabarica (L.) R. BR. LEAF EXTRACT AND ITS ANTIBACTERIAL ACTIVITY

Abstract

The development of new antimicrobial materials has arisen in response to the need to prevent and control the growth of pathogenic microorganisms without the use of antibiotics. The Silver (Ag) nanoparticles attached r-GO (rGO/Ag) nanocomposite was synthesised in the present work using a one-pot in-situ chemical reduction approach and studied for antibacterial property. We developed a rapid preparation of graphene from graphite using modified Hummers method and reduced by A. malabarica leaf extract. UV-Vis spectroscopy and FT-IR were used to characterize the synthesized GO and rGO–Ag nanocomposite. The antibacterial properties of the synthesized GO and rGo-Ag nanocomposite were evaluated against human pathogenic organisms like Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae and Staphylococcus aureus using agar well diffusion method. At 50µl concentrations, synthesised GO inhibited Pseudomonas aeruginosa (19 mm) and rGO-Ag nanocomposite inhibited Staphylococcusaureus (28 mm).Antibacterial properties of the nanocomposites were found to be efficient against both gram positive and gram negative bacteria. Thus, green synthesized RGO-supported Silver nanoparticles play an important role in the medical disciplines.