Green synthesis of polyacrylamide grafted Neem Gum for gastro retentive floating drug delivery of Ciprofloxacin Hydrochloride: In vitro and in vivo evaluation

Abstract

Gastro retentive Floating Drug Delivery Systems (GFDDS) are typically designed to enhance the efficiency of drugs; absorbed in the stomach and possess a short elimination half-life by controlling the drug release rate. This study presents, that Acrylamide Grafted Neem gum (AAm-g-NG) was synthesized for the development of tablet-based GFDDS by using the Microwave-assisted technique for the delivery of Ciprofloxacin Hydrochloride (CFX.HCL). Formulations were evaluated for pre-and post-compression studies like bulk, tapped density, angle of repose and hardness, friability, drug content, weight variation, and drug release, which satisfied all the official requirements of the tablet dosage form. The Structural characterization of the synthesized AAm-g-NG was studied by various analytical techniques like NMR, DSC, FTIR, XRD, and SEM. AAm-g-NG showed a significant swelling index, Water Retention Capacity, Waterlogged capacity, viscosity, and in vitro buoyancy compared to NG and HPMC K100 M. AAm-g-NG was also studied for hemolysis and cytotoxicity; found to be biodegradable and exhibited no toxicity in the Caco-2 cell line. The optimized batch F5 showed controlled drug release of 89% ± 3.56 at 12 h in pH 1.2 and in vivo gastric retention was approx. 8 h in rabbits. The ability of AAm-g-NG graft copolymer to extend the drug release with enhanced gastric retention is well supported by in vivo buoyancy studies and its satisfactory physicochemical properties make it a promising vehicle for gastro retentive system.