Abstract
Nano hydroxyapatite (nHA) was synthesized from different methods. Spherical-like shaped nanoparticles of nHA were synthesized by chemical precipitation using Euclea natalensis root extract as a template for the first time. In addition, traditional synthesis was performed, obtaining rod-like nanoparticles. The formation of nHA was confirmed using Fourier Transform Infrared (FTIR), X-Ray Diffraction (XRD), and Transmission Electron Microscopy (TEM). HA from green route presented a spherical-like shape with smooth surface. However, nanogrooves covered over the surface of nHA obtained without green template. Additionally, the cytotoxic effect of the nHA nanoparticles on L929 viability was assessed using MTS assay. The particles exhibited less cellular toxicity at shorter incubation periods. Spherical-like and rod-like nHA particles interact differently with fibroblast after 24 h of evaluation.
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