Green synthesis of magnetic Fe3O4/Ag nanocomposite using Pomegranate peel extract for the treatment of ovarian cancer

Abstract

In this work, we have reported the biogenic supported silver nanoparticles over surface of magnetic Fe3O4 nanoparticles by using Pomegranate peel extract. The extract was used as a green reducing agent and an excellent stabilizer of the synthesized Ag NPs. Physicochemical characterization of the as-synthesized Fe3O4/Ag NPs was carried out through electron microscopy (SEM and TEM), energy dispersive X-ray spectroscopy (EDX), elemental mapping (WDX), vibrating sample magnetometer (VSM), X-ray diffraction (XRD) and inductively coupled plasma-optical emission spectroscopy (ICP-OES). Theanti-ovarian cancer effects of biologically synthesized Fe3O4/Ag NPs against ovarian cancer cell lines were assessed. The anti-ovarian cancer properties of the Fe3O4/Ag NPs could significantly remove NIH: OVCAR-3‎, ES-2‎, TOV-21G cancer cell lines in a time and concentration-dependent manner by MTT assay. The antioxidant activity of Fe3O4/Ag NPs was determined by DPPH method. The Fe3O4/Ag NPs showed the high antioxidant activity according to the IC50 value. It seems that the anti-human ovarian cancer effect of recent nanoparticles is due to their antioxidant effects. After clinical study, Fe3O4/Ag NPs bio-composite can be utilized as an efficient drug in the treatment of human ovarian cancer in humans.