Green synthesis of gold nanoparticles using Clitoria ternatea: in-vitro and in-silico antioxidant activity of its phytochemicals

Abstract

In the present work, we synthesized gold nanoparticles (AuNPs) using Clitoria ternatea (CT) leaf extract, which was characterized by using analytical techniques, and antioxidant activity was estimated by both (DPPH) and reducing power assay. p-Hydroxycinnamic acid, Quercetin, Kaempferol, and Myricetin were selected from CT leaf extract and molecular docking was performed against antioxidant enzymes SOD and GPX. From the results, the AuNPs synthesized were confirmed by the color change, shape, size, and purity. These AuNPs showed the greatest inhibition (68.3%) in the DPPH and absorbance (0.832 a.u) in the reducing power assay. From these results, it was concluded that the synthesized gold nanoparticles are stable and has antioxidant properties, the phytochemicals selected from CT leaf extract also have potent interaction with two proteins that can be used as therapeutics. Based on in-silico properties of the ligands, the p-Hydroxycinnamic acid showed the best drug-like properties when compared to other ligands.