Abstract

In this paper, we report the synthesis of gold nanoparticles (BPAuNPs) using banana pith extract. Biosynthesized BPAuNPs were screened for phytochemicals coated over them and further characterized by UV-visible spectroscopy, FTIR, XRD and particle size analyser. UV-Visible spectrscopic analysis confirmed the production of BPAuNPs at 530 to 560 nm, where the colour change in the solution from light yellow to deep purple indicated the formation of BPAuNPs. FT-IR analysis confirmed the capping of BPAuNPs with organic residues like, proteins, amino acids and polyphenols present in the extract, which led to stabilization of BPAuNPs. Negative zeta values indicated the stability of BPAuNPs. XRD results prooved the crystalline nature of BPAuNPs. The synthesized BPAuNPs were tested for their antibacterial activity against both Gram positive (Bacillus subtilis) and negative (E.coli, Pseudomonas aeruginosa) bacteria with known antibiotic as control. BPAuNPs showed significant bacteriostatic effect. The BPAuNPs were found to have a positive catalytic activity of reduction of malachite green dye, which was confirmed by the time dependent reduction in absorbance maxima, it can be ascribed to the electron relay response.

Highlights

  • Synthesis of nanoparticles (NPs) using biological materials has gained importance in recent years because it is cost effective, environmental friendly, easy scale up ability for large scale synthesis and further, there is no need to use high pressure, energy, temperature and toxic chemicals (Jayseelan et al ., 2013)

  • We report the synthesis of gold nanoparticles (BPAuNPs) using banana pith extract as banana stem is available throught the year and is usually discarded as a waste material (Li et al, 2010; Nguyen et al, 2017)

  • Biosynthesized BPAuNPs were characterized by UV-visible spectroscopy, FTIR, XRD and Particle size analyser

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Summary

Introduction

Synthesis of nanoparticles (NPs) using biological materials has gained importance in recent years because it is cost effective, environmental friendly, easy scale up ability for large scale synthesis and further, there is no need to use high pressure, energy, temperature and toxic chemicals (Jayseelan et al ., 2013). There are reports in the literature suggesting that the electrostatic attraction of negatively charged bacterial cell membrane and positively charged nanoparticles is crucial for the antibacterial activity of the nanoparticles (Edison et al, 2012). Nanoparticles synthesized using biological materials can be used for bioremediation of environmental pollutants like textile dyes (Arunachalam et al, 2012; Xin et al, 2016).Iron nanoparticles synthesized using Guanyin tea extract have been used for degradation of bromothymol blue (Xin et al, 2016). We report the synthesis of gold nanoparticles (BPAuNPs) using banana pith extract as banana stem is available throught the year and is usually discarded as a waste material (Li et al, 2010; Nguyen et al, 2017). The synthesized BPAuNPs were evaluated for catalytic activity by Malachite green dye reduction test

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