Green synthesis of bovine serum albumin/oxidized gum Arabic nanocomposite as pH-responsive carrier for controlled release of piperine and the molecular docking study

Abstract

A safe drug carrier was synthesized by albumin (BSA) and oxidized gum arabic (OGA). Piperine (PIP) was loaded into BSA/OGA nanobiocomposites by desolvation method. A set of experiments were designed by considering different contents of OGA (5, 7.5 and 10 mg) and PIP (1 and 2 mg). The presence of the band at 1600–1660 cm−1 in FTIR spectra revealed the successful interaction between OGA and BSA. PIP2-BSA/OGA5 was selected as a suitable carrier due to its smaller size (<300 nm) and higher loading efficiency (1.5 ± 0.2 %). The encapsulation efficiency of PIP into BSA/OGA5 was 57.6 ± 2 %. The average size, polydispersity index and zeta potential of PIP2-BSA/OGA5 were 292 ± 4.4 nm, 0.185 ± 0.03 and − 24.4 ± 1.7 mV, respectively. SEM and TEM images proved the formation of spherical-shaped nanoparticles. The disappearance of endothermic peak belonging to free PIP in DSC thermogram of PIP2-BSA/OGA5 evidenced its encapsulation into carrier. PIP2-BSA/OGA5 exhibited the sustained drug release. The cell viability of MCF-7 cells after 48 h exposure to BSA/OGA5, PIP2-BSA/OGA5 and free PIP was reported 90 %, 40.1 % and 30.6 %, respectively. The molecular docking study reported that the binding affinity of PIP for BSA/OGA nanocomposite was −8.7 kcal/mol indicating the acceptable stability of the prepared drug carrier.