Green Synthesis of Bioconjugated Magnetic Nanoparticles (MNPs) By Punica granatum And Its Applications As Anti-microbial Agent

Abstract

Abstract Introduction In recent years, the development of efficient green chemistry methods for synthesizing metal nanoparticles has become a major focus of many researchers. Nanoparticles produced by plants are more stable, and the rate of synthesis is faster than in the case of microorganisms. Methods/Case Report Objectives: Synthesis of (MNPs) by Punica granatum, confirming the formation that it exists, and studying their antibacterial activity effects. Materials and methods By using the Pomegranate plant, drying the plant 200 g (hot oven/sun), grinding the plant (powder form), dissolving in methanol 800 ml, leaving it overnight, filtration with filter paper, then collecting the filtrate, and adding equal volumes of iron solution in a beaker. We observed color change due to nanoparticle formation. By using UV - visible spectroscopy, we proved the presence of nanoparticles manufactured using pomegranate. After that, we studied its antibacterial effect by using several concentrations to determine the least effective (MIC) concentration against the bacteria Staphylococcus aureus on Mueler Hinton agar media. Results (if a Case Study enter NA) The nanoparticles were formed strongly and remarkably, and the color quickly changed to an intense black color, even before using the magnetic mixer. The UV - visible spectroscopy characterizes the synthesized Fe3O4 (MNPs). The peak was at 410 nm, and the black color was developed to indicate the presence of (MNPs). Then we performed antibiotic testing on Staphylococcus aureus by using several concentrations. The nanoparticles were highly effective by using the full concentration and effective by using 1/2, 1/4, 1/8 concentrations. But it was inactive when the concentration was 1/16. Conclusion Nanotechnology is a modern technique to perform an accurate and reliable method for diagnosing and treating many diseases. The magnetic nanoparticles coated with the active ingredients of pomegranate extract facilitate its use the use of them as an antibacterial agent.