Abstract
In this study, eco-friendly, non-toxic gold nanoparticles (AuNPs) were synthesized by green technique using a novel biodegradable graft copolymer based on hydroxy ethyl starch (HES) and methylacrylate (MA). The graft copolymer was prepared by first grafting of MA onto HES backbone by solution polymerization technique using potassium persulfate (K2S2O8) as the initiator followed by alkaline hydrolysis. The graft copolymer was characterized by FTIR and 1H NMR spectroscopy and XRD and SEM studies and by measuring saponification equivalent (S.E.). Biodegradation of the graft copolymer was studied by enzyme hydrolysis. AuNPs prepared by this method were characterized by UV–VIS spectroscopy and XRD, HR-TEM and EDAX analyses. The formation of AuNPs was confirmed by a visual color change (colorless to purple) and appearance of SPR band at 529nm. The prepared AuNPs are globular in shape with an average particle size of 16–20nm. Antibacterial activity of the green synthesized AuNPs was studied against two gram negative bacteria namely Shigella flexneri and Proteus mirabilis and two gram positive bacteria namely Bacillus cereus and Bacillus subtilis by agar well diffusion method. AuNPs synthesized by using the graft copolymer had a significant antibacterial effect against gram positive bacteria at very low concentration of nanoparticles.
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