Green Synthesis, Characterization and Antibacterial Activity of Copper Nanoparticles from the Roots Extract of Piliostigma Reticulata Fabaceae

Abstract

Nanotechnology is a fast-growing area in science which is an interdisciplinary field of both science and technology. In this study, the Roots extracts of Piliostigma reticulata were used for the green synthesis of Copper (Cu) nanoparticles (NPs) from copper Sulphate solution. Green synthesized metallic nanoparticles were characterized by UV– visible spectrophotometer, X-ray diffraction (XRD), Fourier Transform Infra-Red spectrophotometer (FTIR) and Scanning Electron Microscope (SEM). Using the Debye Scherrer formula, the average crystalline size of CuNPs was calculated as 51.0nm respectively. The UV–Vis spectroscopy results showed Surface Plasmon Resonance peaks at 500nm respectively. Fourier transform infrared spectroscopy was measured to identify the possible functional group of the biomolecules in the extract that act as reducing and capping agents of the nanoparticles. SEM analysis describes surface morphology of the synthesized nanoparticles which were roughly spherical in shape. The synthesized Nanoparticles showed antibacterial activity against pathogenic bacteria (Escherichia coli and Staphylococcus aureus) and the highest antibacterial activity was found in Cu-NPS compared plant root extract.