GREEN SYNTHESIS AND EVALUATION OF SILVER NANOPARTICLES USING CYPERUS ROTUNDUS

Abstract

The study focuses on the green synthesis of silver nanoparticles using plant extract of Cyperus Rotundus as reducing, capping and stabilizing agent. Aqueous silver ions when exposed to plant tubers extract [Cyperus Rotundus] resulted in a color change indicating the formation of silver nanoparticles. The synthesized silver nanoparticles were characterized by UV-Vis spectrophotometer, Transmission Electron Microscopy (TEM) and Fourier Transform Infra-red Spectroscopy (FTIR) techniques. The UV-Vis spectra showed peak at 455 nm. TEM revealed that the synthesized silver nanoparticles are spherical and circular in morphology and ranges between 15nm to 100 nm. The particle size and zeta potential range of AgNPs monitored by particle size analyzer Mastsizer 2000 and result was found to be 15nm and -9.03mV. The plant extract of Cyperus Rotundus possess wound healing property which may further be potentiated in the presence of silver nanoparticles. Synthesized silver nanoparticles were incorporated into gel base and different formulations were prepared from F1 to F4 having different carbopol concentration, out of which F2 was found to be optimal and further evaluated for its physical properties such as pH, viscosity & spreadability. The results obtained in the developed formulation showed no lumps, had uniform color dispersion and were free from any fiber and particle. It was also observed to have easy wash ability, good spreadability. Hence, silver nanoparticle of Cyperus Rotundus in aqueous gel-base can be used as an appropriate formulation for Wound Healing.