Abstract
Nanoparticles (NPs) are, frequently, being utilized in multi-dimensional enterprises. Silver nanoparticles (AgNPs) have attracted researchers in the last decade due to their exceptional efficacy at very low volume and stability at higher temperatures. Due to certain limitations of the chemical method of synthesis, AgNPs can be obtained by physical methods including sun rays, microwaves and ultraviolet (UV) radiation. In the current study, the synthesis of pullulan mediated silver nanoparticles (P-AgNPs) was achieved through ultraviolet (UV) irradiation, with a wavelength of 365 nm, for 96 h. P-AgNPs were formed after 24 h of UV-irradiation time and expressed spectra maxima as 415 nm, after 96 h, in UV-vis spectroscopy. The crystallographic structure was “face centered cubic (fcc)” as confirmed by powder X-ray diffraction (PXRD). Furthermore, high resolution transmission electron microscopy (HRTEM) proved that P-AgNPs were covered with a thin layer of pullulan, with a mean crystalline size of 6.02 ± 2.37. The average lattice fringe spacing of nanoparticles was confirmed as 0.235 nm with quasi-spherical characteristics, by selected area electron diffraction (SAED) analysis. These green synthesized P-AgNPs can be utilized efficiently, as an active food and meat preservative, when incorporated into the edible films.
Highlights
Metal nanoparticles (NPs) have been frequently investigated by the researchers for their multi-dimensional applications after their synthesis in 1959 by Richard Feynman, with a size ranging from 1 nm to 100 nm, [1,2,3]
The recent trend of nanotechnology research has been diverted to antimicrobial food packaging, delivery of nano-medicines/drugs, gene delivery vectors, nano-imaging and Biosensors for cancer diagnosis, and polymeric nano-composite wound dressing [3,4]
Pullulan was used as a “green” capping, stabilizing and reducing agent without any addition of toxic or harmful chemicals
Summary
Metal nanoparticles (NPs) have been frequently investigated by the researchers for their multi-dimensional applications after their synthesis in 1959 by Richard Feynman, with a size ranging from 1 nm to 100 nm, [1,2,3]. NPs exhibited a new behavior of efficiency at even very low concentration [4]. The recent trend of nanotechnology research has been diverted to antimicrobial food packaging, delivery of nano-medicines/drugs, gene delivery vectors, nano-imaging and Biosensors for cancer diagnosis, and polymeric nano-composite wound dressing [3,4]. Among metal NPs, the utilization of silver nanoparticles (AgNPs) in the biomedical sector, animal and human nutrition, electronic industry and food preservation is remarkable [5]. AgNPs, as compared to other metal NPs, exhibit very low volatility, with tolerance against high temperatures as compared to other nanoparticles [6]. AgNPs can restrain the growth of the microorganisms (including Gram positive and Gram negative bacterial strains; fungi) from the initial contact and further penetration into the cellular membrane of the microbes [7,8]
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