Green synthesis and characterization of chitosan doped nickel oxide nanocomposites using Euphorbia hirta and its antimicrobial activity

Abstract

Chitin is extracted from shells of shrimps and characterized using UV-drs spectroscopy.It is converted to chitosan by the process of deacetylation and to nanochitosan by the process of sonication.Chitosan and nanochitosan are characterised using UV-Vis and FTIR spectroscopy. Nickel oxide nanoparticles (NiO) are green synthesised from Nickel chloride crystals using Euphorbia hirta medicinal plant leaf extract which contains alkanes, triterpenes, phytosterols, tannins, polyphenols and flavanoids as reducing agent. E.hirta serves as a good medicine for treating female disorders, respiratory ailments, worm infestations in children, dysentery, jaundice, pimples, gonorrhea, digestive problems and tumors.The formation of nano NiO is characterised by UV-Vis, FTIR and XRD spectroscopic techniques. The nano NiO particles are doped with nanochitosan and the antimicrobial activity of the resulting composite is analysed with Staphylococcus aureus and Pseudomonas aeruginosa bacterium using amikacin as the control. The results shows the enhancement in the antibacterial nature of the composite.