Green synthesis and antimicrobial evaluation of silver nanoparticles mediated by leaf extract ofSyzygium cuminiagainst poultry pathogens

Abstract

The present study was carried out to determine the effect of plant extract of Syzygium cumini and their silver nanoparticles (AgNPs) against the poultry pathogens. The plant extract was prepared in methanol, ethanol, ethyl acetate, chloroform, and n-hexane. The results of phytochemical screening showed that the leaf extract of S. cumini have medicinally important phytochemical constituents. In the present study, AgNPs were synthesised by using an extract of S. cumini. Synthesised AgNPs were confirmed by Fourier-transform infrared spectroscopy, ultraviolet–visible, and scanning electron microscopy. A surface plasmon resonance peak in absorption spectra showed absorption at 419 nm. The synthesised nanoparticles were found spherical in shape with the diameter range of 30–120 nm. The antimicrobial activity of crude extract and AgNPs was checked against the poultry bacterial pathogens by the agar well diffusion method. The authors’ findings revealed that the crude extract and AgNPs have promising activities against all the test pathogens. It is concluded that the bioactive compounds from the extract of S. cumini and extract-mediated AgNPs can be significantly used as an alternate poultry medicine to replace the available antibiotics, which have adverse side effects.