Abstract
In this study, extraction of soluble proteins from rapeseed cake using different conventional and innovative extraction processes in order to maximize the extraction yield has been investigated. Firstly, various extraction techniques including ultrasound, microwave, and percolation were tested to increase the protein recovery efficiency. Secondly, response surface methodology (RSM) using a central composite design (CCD) approach was applied to investigate the influence of process variables on ultrasound-assisted extraction (UAE). Statistical analysis revealed that the optimized conditions providing a protein yield of 4.24 g/100 g DM were an ultrasound power of 5.6 W·cm−2 and temperature of 45 °C. Quantitatively UAE followed by two stages of conventional extraction gave the best total protein yield of 9.81 g/100 g DM. Qualitatively, the protein efficiency ratio (PER) used as measure of the nutritive value (12S/2S ratio) which indicates protein quality in terms of S-containing essential amino acids, was similar to that of the conventional extraction method. Small amounts of protein aggregate were observed in the HPLC profile of the extract.
Highlights
Proteins, along with carbohydrates and lipids, are one of the three macronutrient families [1].They consist of a sequence of 20 amino acids (AA) of which eight are considered as essential and that must be provided by food [2]
An optimal yield of 8.58 ± 0.05 g/100 g DM was obtained by using percolation in only 45 s of extraction vs. 8078.58 ± 0.05 g/100 g for ultrasound assisted extraction
Results were obtained by mg of Bovine Serum Albumin (BSA)/100 g DM and were converted in g of proteins/100 g of DM by using the multiplication factor of 0.25
Summary
Along with carbohydrates and lipids, are one of the three macronutrient families [1] They consist of a sequence of 20 amino acids (AA) of which eight are considered as essential (i.e., not synthesized by humans) and that must be provided by food [2]. Alkaline extraction with sodium hydroxide is the most widely used method to recover protein isolates from various matrices. This procedure is generally followed by a precipitation step by addition of a diluted acid. Different protocols will be investigated to optimize the more valuable extraction process
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