Green recovery and application of berry anthocyanins in functional gummies: Stability study, plasma and cellular antioxidant and anti-inflammatory activity

Abstract

This study investigates the comprehensive effects of extraction parameters, freeze-drying, and formulation on the chemical composition, colour properties, antioxidant and anti-inflammatory activities, and reactive oxygen species (ROS) generation of blackberry (BB) and elderberry (EB) extracts, as well as their incorporation into gummies. Using response surface methodology, optimal extraction conditions were identified: BB extracts showed optimal results at 325 W and 7.5 min, while EB extracts were optimal at 400 W and 5 min. The EB extracts consistently exhibited higher total phenolic content, total anthocyanin content, and antioxidant capacity than the BB extracts. Over 120 min, BB extracts demonstrated superior antioxidant potential to mitigate human plasma lipid oxidation. Both extracts displayed pH-dependent colour variations and antioxidant capacities, with EB extracts showing greater stability across a broader pH range. Freeze-drying effectively preserved antioxidant capacity, with EB extracts maintaining higher values than BB extracts. In a cellular model of oxidative stress using THP-1, both extracts were non-cytotoxic and reduced intracellular ROS generation, with EB extracts also more effectively inhibiting IL-6 secretion. When incorporated into gummies, these extracts resulted in higher phenolic and anthocyanin content than commercial counterparts, with EB gummies demonstrating superior antioxidant capacity. Sensory evaluations indicated no significant differences in taste, texture, or overall acceptability among the gummy formulations, though colour preferences tended to favour commercial gummies. This study addresses a gap by providing detailed chemical, biological, and sensory assessments of BB and EB extracts in food applications.