Abstract
AbstractWe developed a green infrared‐assisted extraction method for the determination of 3′‐hydroxy puerarin, puerarin, daidzin, genistin, and daidzein in Pueraria lobata. After a simultaneous optimization of Box–Behnken design and desirability function, the developed method provided better isoflavone extraction efficiencies with a lower solvent consumption and shorter extraction time than other methods. The optimized extraction used an extraction time of 8 min, solid–liquid ratio of 1:100, and ethanol volume fraction of 60%. Additionally, ultra‐high performance liquid chromatography was used for identification and quantification of the five isoflavones. Our method showed good linearity (r >0.999) over wide concentration ranges. The ranges for the limits of detection and quantitation were 0.03–0.09 μg/mL and 0.14–0.44 μg/mL, respectively, the recovery range was 98.38–103.37%, and the run time was 5.5 min. This approach is efficient, economical, and eco‐friendly, and shows promise for rapid extraction and quantitative analysis of bioactive isoflavones from Pueraria lobata.
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
