Abstract

Important green house gases (GHG) attributed to animal agriculture are methane (CH 4 ) and nitrous oxide (N 2 O), though carbon dioxide (CO 2 ) contributes almost half of total greenhouse effect. Rumen CH 4 production in an enteric fermentation can be accounted as the biggest anthropogenic source. Some of prebiotics and probiotics have been innovated to mitigate rumen CH 4 emission. The possible use of agricultural biomass consisted of non-edible parts of crop plants such as cellulose and hemi cellulose and animal wastes was proposed as a renewable energy and nitrogen sources. The ammonia stripping from digested slurry of animal manure in biogas plant applied three options of nitrogen recycling to mitigate nitrous oxide emission. In the first option of the ammonia stripping, the effect of ammonolysis on feed value of cellulose biomass was evaluated on digestibility, energy metabolism and protein utilization. Saccharification of the NH 3 treated cellulose biomass was confirmed in strictly anaerobic incubation with rumen cellulolytic bacteria, Ruminoccous flavefaciens , to produce bio-ethanol as the second option of ammonia stripping. In an attempt of NH 3 fuel cell, the reformed hydrogen from the NH 3 stripped from 20 liter of digested slurry in thermophilic biogas plant could generate 0.12 W electricity with proton exchange membrane fuel cell (PEM) as the third option. Key words: GHG, rumen, methane, probiotics, ammonia stripping, biomass

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