Green fluorescent protein-doped sol-gel silica planar waveguide to detect organophosphorus compound

Abstract

We report novel living protein-doped planar waveguide, and real-time detection of an organophosphorus compound using a sol-gel silica planar waveguide doped with a green fluorescent protein and an organophosphorus hydrolase on a yeast-cell surface. The waveguide was pumped at 488 nm, and emitted green fluorescence at the far field. The green fluorescent light at 550 nm changed by 50% from the original power 1 min after application of the organophosphorus compound. The results enable the real-time detection of biochemical weapon and insecticide harmful for human body by using an in-line fiber sensor network.