Abstract
A green, cost-effective, and simple capillary zone electrophoresis (CZE) method was developed and validated for simultaneous determination of chloramphenicol, methylparaben, and propylparaben in eye-drops. With sodium tetraborate as background electrolyte (BGE), the apparent mobilities of chloramphenicol, methylparaben, and propylparaben increased and analysis time reduced when pH of BGE increased from 8.5 to 10.0 and concentration of BGE decreased from 40 mM to 15 mM, but complete separation of chloramphenicol from other matrix components was achieved only with sodium tetraborate concentration at 30 mM or higher and at pH = 9.3 or lower. The most suitable electrophoretic conditions for the intended application were a 30 mM sodium tetraborate solution, pH 9.3 as BGE, working voltage set at 25 kV, and UV detection at 280 nm at the cathodic extremity of the capillary. The final method was validated and proved to be reliable for assay of chloramphenicol, methylparaben, and propylparaben in eye-drops.
Highlights
Chloramphenicol is a broad-spectrum antibiotic effective against both Gram-positive and Gram-negative bacteria [1]
UV-Vis spectroscopy has been used for assay of chloramphenicol in pharmaceutical-grade raw material [5]; the high-performance liquid chromatography (HPLC)
Method using C18 column has been developed for the assay of chloramphenicol in pharmaceutical dosage forms [5] and biological sample [6]; and capillary zone electrophoresis (CZE) with amperometric detection has been used for quantifying chloramphenicol in blood [7] and eye-drops [8]
Summary
Chloramphenicol is a broad-spectrum antibiotic effective against both Gram-positive and Gram-negative bacteria [1]. In pharmaceutical preparations, only methylparaben and propylparaben are currently used as preservatives due to concerns regarding their safety [4]. Method using C18 column has been developed for the assay of chloramphenicol in pharmaceutical dosage forms [5] and biological sample [6]; and capillary zone electrophoresis (CZE) with amperometric detection has been used for quantifying chloramphenicol in blood [7] and eye-drops [8]. Propylparaben and methylparaben have been analyzed simultaneously in cosmetic products with reversed-phase HPLC [10], in pharmaceutical and cosmetic products with capillary electrophoresis with conventional UV detection [11], and in pharmaceutical preparations by microchip electrophoresis with conductivity detection [12].
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