Green coalescence of CuO nanospheres for efficient anti-microbial and anti-cancer conceivable activity

Abstract

In this study, copper oxide nanospheres (CuO-NSs) were synthesized using Long pepper (Piper longum) extract. The biomolecules present in Piper longum extract are mainly responsible for reducing metal ions to metal nanoparticles. The synthesized CuO-NSs were characterized using SEM (scanning electron microscopy), TEM (transmission electron microscopy), XRD (X-ray diffraction), and UV-Vis spectroscopic techniques. SEM and TEM images indicated that the as-prepared CuO-NSs were floral spherical nanospheres shaped with a size distribution ranging from 4 to 400 nm. Besides, the synthesized CuO-NSs have shown crucial biological activity against pathogenic bacterial strains, viz. Klebsiella pneumonia, Bacillus subtilis, and Escherichia coli. The antimicrobial activity of CuO-NSs was scrutinized by an exemplary disk diffusion assay and minimum inhibitory concentration of CuO-NSs against various strains of bacteria. Furthermore, as-synthesized CuO-NSs was also applied as a potential material for hemolysis activity and determined its cytotoxicity effect on A549 cells. CuO-NSs has been tested in A549 cells, and the results of different characterization techniques confirmed that the synthesized CuO-NSs exhibited unique optical, morphological, antibacterial, and anticancer behaviors. The study elucidates the Piper longum effectiveness on uncontrolled cell division parameters, and different cancer reductions from in-vitro analysis. The Long pepper (Piper longum) extract with CuO-NSs could significantly stimulate carcinogenic conditions because of discrete standards that penetrate the target cells and good potential against the bacterial strains.