Green Approach for Sample Preparation and Determination of Anthocyanins from Lycium ruthenicum Murr. Using a β-Cyclodextrin-Based Extraction Method Coupled with UPLC-DAD Analysis

Abstract

This study aimed to develop and optimize a β-cyclodextrin (β-CD)-based technique for extracting anthocyanins from Lycium ruthenicum Murr. and to establish a ultra-high-performance liquid chromatography-diode array detector (UPLC-DAD) method for their analysis. β-CD solutions produced higher extraction yields of petunidin-3-O-(trans-p-coumaroyl)-rutinoside-5-O-glucoside and total anthocyanins from L. ruthenicum fruit than did pure water and aqueous hydroxypropyl-β-cyclodextrin (HPβ-CD) and ethanol and methanol solutions. Extraction at 50 °C for 30 min using 1.65% β-CD solution and a liquid/solid ratio of 15:1 produced the optimal extraction yield of L. ruthenicum anthocyanins. A UPLC-DAD method was developed for the determination of L. ruthenicum anthocyanins using an ethanol-based mobile phase, and the primary anthocyanins were identified using two-dimensional LC-MS/MS. Method validation showed that the developed method was accurate, stable, and reliable for the analysis of petunidin-3-O-(trans-p-coumaroyl)-rutinoside-5-O-glucoside and total anthocyanins from L. ruthenicum fruit. The present study showed that β-CD-based extraction coupled with UPLC-DAD analysis is an efficient and green method for the extraction and analysis of anthocyanins from L. ruthenicum fruit.