Abstract
Carbon dioxide is a useful anesthetic for immobilizing insects in the laboratory, but several studies have demonstrated effects beyond anesthesia. It was found to enhance the activity of some fumigants (AliNiazee and Lindgren 1969). Also, 20 min of exposure to CO2 anesthesia was found to have significant deleterious effects on some subsequent biological activities of adult codling moths, Laspeyresia pomonella (L.) (White et al. 1970), as did 30 min of exposure (but not 15 min) on adult Mediterranean fruit flies, Ceratitis capitata (Wiedemann) (Hooper 1970). Therefore, several tests were conducted with grasshoppers at the Bozeman laboratory to determine any possible interaction between CO2 anesthesia and malathion and the effect of CO2 anesthesia on growth and development. Malathion is currently used as the check in screening new insecticides at this laboratory.
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