Abstract
Two graphical rules for calculating the reaction rates of steady-state enzyme-catalysed systems are presented. According to Rule 1, the number of non-zero terms can be easily calculated, which is useful to check the results and avoid mistakes. Rule 2 provides a new graphical method, which will be much more effective than the existing methods in dealing with complicated problems. In addition, during the process of deriving Rule 1, the mathematical principles of the Wong-Hanes structural rule have been naturally obtained too.
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