Abstract
We engineered an ingenious fluorescent aptasensor for detection of Pseudomonas aeruginosa ( P. aeruginosa) according to the DNA hybridization and fluorescence resonance energy transfer. In the absence of target bacteria, 5-carboxyfluorescein-labeled complementary DNA (FAM-cDNA) hybridizes with the partial sequences of aptamer and the fluorescence of FAM can be quenched by graphene oxide quantum dots (GOQDs). Upon the addition of target bacteria, the aptamer as a biorecognition element is bound with P. aeruginosa specifically. FAM-cDNA prefers to hybridize with the aptamer, resulting in the desorption of FAM-cDNA from GOQDs, thus recovering the fluorescence of FAM. The aptasensor shows a wide linear response to P. aeruginosa in the concentration range of 1.28 × 103-2.00 × 107 cfu/mL with acceptable selectivity. The detection limit is 100 cfu/mL. The whole process can be finished in 2 h. Moreover, the platform is successfully applied to detect P. aeruginosa in drinking water, orange juice, and popsicle samples.
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