Abstract
The monitoring of antibiotic residues in foodstuffs by using rapid detection method is essential for food safety. In this work, the electrochemical sensor was developed by modification of screen-printed carbon electrode with graphene oxide, and then the ciprofloxacin (CIP) was detected based on the complexation of CIP with Mn2+. On modified electrode, the anodic stripping peak current response of Mn2+ was prohibited in the presence of CIP, and a peak current response of the complex was occurred. Thus, the peak current response of the complexation peak was employed as the indicating signal for CIP determination, which was more sensitive than the direct electrochemical oxidation response of CIP. Parameters that affect the signal response have been investigated in method. Under the optimum conditions, the peak current of the complexation peak was linearly correlated with the CIP content in the milk sample solution at 1.0 to 8.0 μM, and the linear correlation coefficients (R2) was 0.994. The limits of detection (LOD) was 0.30 μM. Recoveries of CIP in milk sample were ranged from 81.0 to 95.4% with relative standard deviations (RSDs) below 4.6%. The method showed high selectivity and sensitive, good reproducibility, indicated that this method has potential to be applied in CIP residue analysis.
Highlights
The widespread use and abuse of antibiotics contribute to the production of multidrug-resistant microorganisms and unhealthy animal derived foods, which has become a growing threat to public health and a global problem announced by WHO (Zhang et al 2018, 2020; Zheng et al 2019)
A novel method for CIP determination was developed based on monitoring the peak current response of the complexation of CIP with M n2+ by modified electrode
The peak current response under different concentrations of CIP were investigated to ascertain that the peak current response at 0.4 V was caused by the formation of CIP and M n2+ complex, and the results were shown in Fig. 1C and Fig. 1D
Summary
The widespread use and abuse of antibiotics contribute to the production of multidrug-resistant microorganisms and unhealthy animal derived foods, which has become a growing threat to public health and a global problem announced by WHO (Zhang et al 2018, 2020; Zheng et al 2019). For high water solubility, easy migration and poor biodegradability under different pH, the use of CIP has a series of negative effects such as contaminating environment and agricultural products (Aminov 2009). Common techniques for determining antibiotics in environmental and food samples include high performance liquid chromatography (HPLC) coupled with various types of detector (Bekoe et al 2014; Liu et al 2005), spectroscopy (Abdulghani et al 2013), and microbiological assays (Mendez et al 2005).
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