Abstract
Because of unique electrical and structural properties, graphene has attracted widespread attention in biosensing applications. In this paper, a single layer of graphene was grown by chemical vapor deposition (CVD). Using graphene as the electric channel, a graphene field effect transistor (G-FET) biosensor was fabricated and used to detect adenosine triphosphate (ATP) and adenosine. Compared with traditional methods, the G-FET biosensor has the advantages of higher sensitivity and better stability. The sensor showed high performance and achieved a detection limit down to 0.5 pM for both ATP and adenosine. Moreover, the G-FET biosensor showed an excellent linear electrical response to ATP concentrations in a broad range from 0.5 pM to 50 μM. The developed graphene biosensor has high sensitivity, simple operation, and fast analysis speed, which may provide a new feasible direction to detect ATP and adenosine. Healthy sexually mature male laboratory Wistar rats, weighing 180-200 gr (“FSUE “Nursery of laboratory animals “Rappolovo”) and having been placed under quarantine not less than for 14 days, were selected for the experiment.
Highlights
In the past few decades, a great deal of research in new sensing technologies/sensors has focused on surface enhanced Raman spectroscopy (SERS) [1], electrochemistry [2, 3], capacitor sensor, and surface plasmon resonance (SPR) [4, 5]
We developed a field effect transistor (GFET) biosensor using graphene as an electron channel for adenosine and adenosine triphosphate (ATP) detection
The large graphene film was cut into lots of pieces with a size of 9 mm × 9 mm × 50 μm for graphene field effect transistor (G-FET) fabrication
Summary
In the past few decades, a great deal of research in new sensing technologies/sensors has focused on surface enhanced Raman spectroscopy (SERS) [1], electrochemistry [2, 3], capacitor sensor, and surface plasmon resonance (SPR) [4, 5]. These sensors have been widely used for chemical and biomolecule detection, but they are difficult to operate at low cost and be miniaturized for measurement [6]. Compared with traditional adenosine and ATP detection methods, the G-FET sensor has high sensitivity, fast analysis speed, simple operation, and low cost. This biosensor may provide a new approach for the detection of adenosine and ATP
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