Abstract
Chitosan-dispersed graphene nano-flakes were prepared by a chemical route to reduce graphene oxide and dispersed fully in water to form a stable black aqueous solution. The as-prepared graphene nano-flakes were successfully immobilized on glassy carbon electrode to construct a graphene modified electrode. Cytochrome c was adsorbed tightly on the surface of the modified electrode and the direct electron transfer of Cytochrome c was achieved. Cytochrome c on the surface of electrode maintains its bioactivity and shows an enzyme-like activity for the reduction of nitric oxide, displaying a potential application for the fabrication of novel biosensors to sense nitric oxide. This research will enlarge the applications of graphene-based materials in biosensor field.
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