Grape Seed Proanthocyanidin Extract Prevents Ovarian Aging by Inhibiting Oxidative Stress in the Hens.

Xin Lin,Xingting Liu,Yuling Mi,Jian Li,Caiqiao Zhang

doi:10.1155/2018/9390810

Abstract

Oxidative stress is an important inducement in ovarian aging which results in fecundity decline in human and diverse animals. As a potent antioxidant, grape seed proanthocyanidin extract (GSPE) was investigated to ameliorate chicken ovarian aging in this study. Firstly, ovarian antioxidant capacity of hens at different ages (90, 150, 280, and 580 days old) was compared to elucidate its age-related changes. Subsequently, a D-gal-induced (2.5 mg/mL) aging ovarian model was established and the cultured ovarian tissues were treated with GSPE at 5 μg/mL for 72 h to evaluate the putative attenuating effects of GSPE on ovarian aging. Meanwhile, ovaries of D280 (young) and D580 (old) were treated with GSPE for 72 h in culture to verify the protective effects of GSPE on natural aging ovary. The results showed that GSPE could rescue the antioxidant capacity decline by increasing the antioxidase activities and their gene expression in either D-gal-induced or natural aging ovaries. Moreover, GSPE could maintain the homeostasis between cell proliferation and apoptosis in the D-gal-induced and natural aging ovaries, as well as alleviate D-gal-induced nucleus chromatin condensation in the ovarian granulosa cells. In conclusion, GSPE treatment can effectively prevent the ovarian aging process in hens by reducing oxidative stress.

Highlights

In mammals, female reproductive capacity is negatively correlated with age, and the female reproductive system is more rapid to show overt signs of physiologic aging than other body systems [1]
The Hematoxylin and eosin (H&E) staining of ovarian tissues from D90, D150, D280, and D580 hens showed that the number of growing follicles at D580 was markedly lower than that in
No significant difference was detected in reactive oxygen species (ROS) levels between the control group and grape seed proanthocyanidin extract (GSPE) group (Figure 6(b), C). These results suggested that D-gal significantly increased the generation of ROS in ovarian tissues, and this increase could be attenuated by concurrent GSPE supplement

Summary

Introduction

Female reproductive capacity is negatively correlated with age, and the female reproductive system is more rapid to show overt signs of physiologic aging than other body systems [1]. Ovarian aging is thought to be dominated by a gradual decrease in both the quantity and the quality of the oocytes residing within the follicles with increasing age [4, 5]. These decreases lead to the decline in the duration of ovarian functions. The poultry undergoes age-related decline in fecundity. Fast decline appears in the egg production of the laying hens after 480 days of age due to ovarian aging, thereby leading to reduced egg production and commercial value of the laying hens. It is necessary to explore the mechanisms of the deterioration of the laying performance and its countermeasures during the aging process

Methods

Discussion

Conclusion