Abstract
Selection of Salmonellae from granulocytes may result in safe and effective vaccine strains. We demonstrated that the reduced virulence of such strains is limited to the species in which the selection was made. Repeated (sequential) selection of Salmonella enteritidis from chicken granulocytes yielded an avirulent strain for chickens. Repeated (sequential) selection of Salmonella enteritidis from pig granulocytes (neutrophils) yielded a strain that was comparable to the original wildtype strain.
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