Grain-Priming with L-Arginine Improves the Growth Performance of Wheat (Triticum aestivum L.) Plants under Drought Stress

Abstract

Drought is the main limiting abiotic environmental stress worldwide. Water scarcity restricts the growth, development, and productivity of crops. Wheat (Triticum aestivum L.) is a fundamentally cultivated cereal crop. This study aimed to evaluate the effect of grain-priming with arginine (0.25, 0.5, and 1 mM) on growth performance and some physiological aspects of wheat plants under normal or drought-stressed conditions. Morphological growth parameters, photosynthetic pigments, soluble sugars, free amino acids, proline, total phenols, flavonoids, and proteins profiles were determined. Drought stress lowered plant growth parameters and chlorophyll a and b contents while increasing carotenoids, soluble sugars, free amino acids, proline, total phenols, and flavonoids. Soaking wheat grains with arginine (0.25, 0.5, and 1 mM) improves plant growth and mitigates the harmful effects of drought stress. The most effective treatment to alleviate the effects of drought stress on wheat plants was (1 mM) arginine, that increased root length (48.3%), leaves number (136%), shoot fresh weight (110.5%), root fresh weight (110.8%), root dry weight (107.7%), chlorophyll a (11.4%), chlorophyll b (38.7%), and carotenoids content (41.9%) compared to the corresponding control values. Arginine enhanced the synthesis of soluble sugars, proline, free amino acids, phenols, and flavonoids in wheat plants under normal or stressed conditions. Furthermore, the protein profile varies in response to drought stress and arginine pretreatments. Ultimately, pretreatment with arginine had a powerful potential to face the impacts of drought stress on wheat plants by promoting physiological and metabolic aspects.