Grain Quality of Doubled Haploid Lines in Rice (Oryza sativa L.) Produced by Anther Culture

M Fazaa,I El-Rewainy,G Anis,M Yildirim,A El Sabagh,M S Islam,C Barutçular

doi:10.5539/jas.v8n5p184

Abstract

<p>Genetic devlopment to imrove genotypes with high quality is the most important approach of rice. Thus, anther culture technique is one of straight forward approaches for improvement of rice cultivars with good grain quality. Therefore, this investigation aimed to develop some doubled haploid lines (DH) through anther culture technique and evaluate them along with their five parents including two check varieties for some nutritional characteristics. The results indicated that the three mineral element contents (Zn, Mn and Fe) of rice grain were clearly different among genotypes (DH), which implied that genotypic variations might provide opportunities to select for higher mineral element content. Analysis of variance revealed that the differences among genotypes were significant for all studied traits. Highly significant positive correlations were recognized among the studied characteristics. Accordingly, Rice lines with the high nutritional values will use as donors for this trait in rice breeding programs for exploitation and in hybridization. <strong></strong></p>

Highlights

Rice is (Oryza sativa L.) an important crop that feeds more than half of the world’s population
Rice lines with the high nutritional values will use as donors for this trait in rice breeding programs for exploitation and in hybridization
Sixty six doubled haploid lines (DHLs) were obtained, thirty nine DHLs derived from cross No 1, fifteen DHLs derived from cross No 2, four DHLs derived from cross No 3, eight DHLs derived from cross No 4 and prepared for seed multiplication and evaluation

Summary

Introduction

Rice is (Oryza sativa L.) an important crop that feeds more than half of the world’s population. Rice is the staple food of more than half of the world’s population and is mostly consumed as cooked rice as the primary dietary source of carbohydrate and energy. Production of double haploids through anther culture is a rapid approach to homozygosity that shortens the time required for the development of new rice cultivars as compared to conventional methods (Xa & Lang, 2011). Anther culture is an actively developed method of great potential It shortens the breeding term and increases selection efficiency by producing doubled haploid plants from hybrids (Yan et al, 1996). Several lines as donors for different traits can be developed and these selected doubled haploid lines could possess high yield potentiality, better grain quality, nutritional value, blast resistance and stem borer resistance (Draz, 2004). With keeping the above points in view, the objective of the current investigation was to develop new rice genotypes with high nutritional value by using anther culture technique

Plant Materials and Growth Conditions

Plant Sampling and Measurements

Grain Quality and Nutritional Value Traits in the Tested Rice Genotypes

Analysis of Variance for Grain Quality and Nutritional Value Traits

Correlation Coefficients for Nutritional Value and Grain Quality Characters

Conclusion