Abstract
Gradients for isoelectric focusing in the pH range 2–5 were developed by adding acids to commercially available carrier ampholytes (Ampholine, L.K.B.). The effects of current direction, voltage, focussing time, stabilising medium and pH gradient composition were studied to find the optimum focussing conditions. In addition, the electric potential was measured in loco during the run in order to determine the field strength in different parts of the column. Details for the production of suitable acid gradients are given. Using these gradients it was possible to fractionate the R-type vitamin B 12-binding protein of human amniotic fluid into six fractions with isoelectric points between pH 2.3 and 4.2.
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
