Abstract
The coupling of flow programming and gradient elution is proposed as an integrated approach to the general elution problem. A model is developed to describe the process in which the flow-rate and mobile phase composition change with time. Experimental results with two model proteins compare favorably with theoretical calculations. A parametric study, experimental and numerical, illustrates the effect of various characteristic parameters. Since initial flow-rates are low, improved front-end resolution can be expected due to an increase in column efficiency. Since the mobile phase composition also changes with time, a wide range of elution strengths can also be applied to the column. This allows for the elution of species which initially are strongly retained.
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