Abstract
Water-soluble locust bean and guar gum galactomannans were fractionated by a graded ammonium sulphate precipitation technique. Locust bean gum galactomannans yielded five fractions obtained at 20, 30, 45, 80 and 100% saturation with (NH 4) 2SO 4. The yields of the fractions based on the total material recovered after fractionation) were 3.0, 60.0, 23.0, 9.0 and 5.0, respectively. Guar gum galactomannans yielded three fractions obtained at 70, 80 and 100% saturation with (NH 4) 2SO 4. The yields were 31.0, 39.0 and 30.0%, respectively. Monosaccharide and [ 13C]-NMR analyses revealed differences in structural characteristics among samples. Fractions precipitated at relatively low saturation of (NH 4) 2SO 4 had a low degree of galactose substitution and a high frequency of unsubstituted mannose residue pairs. They also showed high limiting viscosity values. Fractions which required much higher concentration of (NH 4) 2SO 4 for precipitation were highly substituted, had low frequency of unsubstituted mannose pairs and relatively low limiting viscosity values.
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